Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: CDK5 downregulation enhances synaptic plasticity

doi: 10.1007/s00018-016-2333-8

Figure Lengend Snippet: Rac inhibition affected CDK5 shRNA-miR-induced spine morphogenesis in mature neurons. a Primary hippocampal neurons were treated with 0.01-, 0.05-, 0.1-, 1-, 2.5-, 5-, 10-, 25-, 50-, and 100-μM NSC23766 for 24 h. After treatment, the LDH released from medium was measured to determine cytotoxicity. b Then, neurons were transduced with Scr or CDK5 shRNA-miR and DIV19 treated with 1- or 5-μM NSC23766 for 30 min to analyze spine morphogenesis. Rac and RhoA activities were quantified for the 30-min NSC23766 treatment as the amount of Rac-GTP and RhoA-GTP corresponding to the optical density observed using ELISA (λ = 490 nm). The data are presented as the mean ± SEM from n = 4 per duplicate. c Morphological characters show: AAV vector viral eGFP-tagged Scr and CDK5 shRNA-miR (green), nuclei visualized with Hoechst staining (blue), and F-actin cytoskeleton visualized with Alexa 594 Phalloidin dye (red). Magnification ×60. Scale bar 20 µm, n = 4. Insets are showing the proximal dendritic shaft for each treatment. Scale bars 2 μm. *p < 0.05; **p < 0.01; ANOVA with Tukey’s test for b

Article Snippet: BDNF assay BDNF release from co-cultures was measured using a mouse anti-BDNF ELISA kit (Promega), as was described previously [ 12 ].

Techniques: Inhibition, shRNA, Transduction, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Staining

Journal:

Article Title: Brain-derived neurotrophic factor (BDNF) contributes to neuronal dysfunction in a model of allergic airway inflammation

doi: 10.1038/sj.bjp.0705638

Figure Lengend Snippet: Animal treatment protocol. Mice were sensitised to OVA adsorbed to Al(OH)3 or vehicle alone by intraperitoneal injections on days 1, 14 and 21. Prior to analysis, animals received two consecutive local aerosol challenges of 1% OVA (w v−1) diluted in PBS or PBS alone and delivered by 20-min aerosolisation on days 26 and 27. Intranasal application of polyclonal chicken IgY (isotype antibody) or anti-mouse BDNF was performed 3 h before each airway allergen challenge. In addition, animals received the antibodies i.p. on day 25. The response to acute allergen exposure was measured on day 35 in the body plethysmograph. All animals were analysed 24 h after the last challenge. Abbreviations: intraperitoneally (i.p.), intranasally (i.n.), aerosol challenge (aerosol).

Article Snippet: Polyclonal chicken IgY anti-mouse BDNF (2 × 25 μ l) (500 μ g ml −1 ) or isotype antibody (500 μ g ml −1 ) (Promega, Madison, U.S.A.) solved in sterile PBS was instilled intranasally 3 h before each airway allergen challenge.

Techniques: Aerosol

Journal:

Article Title: Brain-derived neurotrophic factor (BDNF) contributes to neuronal dysfunction in a model of allergic airway inflammation

doi: 10.1038/sj.bjp.0705638

Figure Lengend Snippet: Overview of experiments and animal numbers

Article Snippet: Polyclonal chicken IgY anti-mouse BDNF (2 × 25 μ l) (500 μ g ml −1 ) or isotype antibody (500 μ g ml −1 ) (Promega, Madison, U.S.A.) solved in sterile PBS was instilled intranasally 3 h before each airway allergen challenge.

Techniques: Aerosol

Journal:

Article Title: Brain-derived neurotrophic factor (BDNF) contributes to neuronal dysfunction in a model of allergic airway inflammation

doi: 10.1038/sj.bjp.0705638

Figure Lengend Snippet: Effect of anti-BDNF treatment on allergen-induced airway obstruction by HBP. Measurement of expiratory flow in response to OVA (intranasal application during continuous measurement of airflow in the HBP). (a) Integration of EF50 values between 15 and 25 min after OVA application. (b) Time course of n=11 animals in each group. Data from three separate experiments are shown. Grey bar: possible artefacts from OVA application. The error bars represent standard error of the mean (s.e.m.). Student́s t-test: ***P<0.001.

Article Snippet: Polyclonal chicken IgY anti-mouse BDNF (2 × 25 μ l) (500 μ g ml −1 ) or isotype antibody (500 μ g ml −1 ) (Promega, Madison, U.S.A.) solved in sterile PBS was instilled intranasally 3 h before each airway allergen challenge.

Techniques:

Journal:

Article Title: Brain-derived neurotrophic factor (BDNF) contributes to neuronal dysfunction in a model of allergic airway inflammation

doi: 10.1038/sj.bjp.0705638

Figure Lengend Snippet: Effect of BDNF and anti-BDNF treatment on inflammation. (a, b) Cytokine levels in BALF. IL-4, IL-5 and IFN-γ were measured in BALF from nonsensitised or OVA-sensitised mice 24 h after aerosol challenge by ELISA. The BAL recovery was 1.4±0.2 ml in all groups. (c, d) Cell differentiation in BALF after OVA aerosol challenge on day 28. Lymphocytes, macrophages, eosinophils and neutrophils were differentiated according to morphological criteria in BALF from nonsensitised or OVA-sensitised mice 24 h after the last aerosol challenge. (e) Cell differentiation in BALF 24 h after nasal OVA challenge on day 36. Student's t-test did not reveal any statistically significant difference (P<0.05) between groups. In all, 12 mice were analysed in each group, in two separate experiments. The error bars represent standard deviation (s.d.).

Article Snippet: Polyclonal chicken IgY anti-mouse BDNF (2 × 25 μ l) (500 μ g ml −1 ) or isotype antibody (500 μ g ml −1 ) (Promega, Madison, U.S.A.) solved in sterile PBS was instilled intranasally 3 h before each airway allergen challenge.

Techniques: Aerosol, Enzyme-linked Immunosorbent Assay, Cell Differentiation, Standard Deviation

Journal:

Article Title: Brain-derived neurotrophic factor (BDNF) contributes to neuronal dysfunction in a model of allergic airway inflammation

doi: 10.1038/sj.bjp.0705638

Figure Lengend Snippet: Effect of BDNF on airway hyper-responsiveness in response to EFS. Airway hyper-responsiveness was measured in response to EFS. The frequency that caused 50% of maximal airway smooth muscle constriction was defined as ES50. Airway contractility was expressed as mean±s.d. Statistics were performed using t-test with Welch's correction. P<0.05 was regarded as a statistically significant difference. (a) Nonsensitised BALB/c mice were treated intranasally either with recombinant human BDNF (5 μg ml−1, 50 μl) or BSA (0.1%, 50 μl) as a control on two consecutive days. AHR was measured 24 h after the last treatment. In all, 12 animals were examined in each group in two separate experiments. (a) OVA-sensitised Balb/c mice were treated intranasally either with anti-BDNF (500 μg ml−1, 50 μl) or istotype IgY (500 μg ml−1, 50 μl) as a control on two consecutive days, 3 h before OVA aerosol challenge. In addition, the animals received 100 μl of the antibody solution i.p. 24 h before the first OVA challenge (day 25). AHR was measured 24 h after the last treatment. A total of 12 animals were examined in each group in two separate experiments.

Article Snippet: Polyclonal chicken IgY anti-mouse BDNF (2 × 25 μ l) (500 μ g ml −1 ) or isotype antibody (500 μ g ml −1 ) (Promega, Madison, U.S.A.) solved in sterile PBS was instilled intranasally 3 h before each airway allergen challenge.

Techniques: Recombinant, Control, Aerosol

Journal:

Article Title: Brain-derived neurotrophic factor (BDNF) contributes to neuronal dysfunction in a model of allergic airway inflammation

doi: 10.1038/sj.bjp.0705638

Figure Lengend Snippet: Effect of anti-BDNF treatment on airway hyper-responsiveness in response to methacholine and capsaicin by HBP. (a) Dose–response curve of expiratory flow (EF50) in response to inhaled methacholine. (b) Dose–response curve of expiratory flow (EF50) in response to inhaled capsaicin. (c) Dose–response curve of sensory irritation (TB) in response to inhaled methacholine. (d) Dose–response curve of sensory irritation (TB) in response to inhaled capsaicin. The error bars represent standard error of the mean (s.e.m.), n=8–10 animals in each group in three separate experiments. Student's t-test: *P<0.05. (a) Nonsensitised against OVA-sensitised+isotype, (d) OVA-sensitised+isotype against OVA-sensitised+anti-BDNF.

Article Snippet: Polyclonal chicken IgY anti-mouse BDNF (2 × 25 μ l) (500 μ g ml −1 ) or isotype antibody (500 μ g ml −1 ) (Promega, Madison, U.S.A.) solved in sterile PBS was instilled intranasally 3 h before each airway allergen challenge.

Techniques:

Journal:

Article Title: Brain-derived neurotrophic factor (BDNF) contributes to neuronal dysfunction in a model of allergic airway inflammation

doi: 10.1038/sj.bjp.0705638

Figure Lengend Snippet: Tachykinin containing sensory neurons from ovalbumin-sensitised mice

Article Snippet: Polyclonal chicken IgY anti-mouse BDNF (2 × 25 μ l) (500 μ g ml −1 ) or isotype antibody (500 μ g ml −1 ) (Promega, Madison, U.S.A.) solved in sterile PBS was instilled intranasally 3 h before each airway allergen challenge.

Techniques:

Journal: Scientific Reports

Article Title: BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells

doi: 10.1038/s41598-020-69307-1

Figure Lengend Snippet: NLC secrete BDNF, thereby protecting B-CLL cells from apoptosis. ( a ) Representative western blots showing expression of BDNF in lysates of NLC from two independent patient samples (n = 5). ( b ) Immunofluorescence analysis of BDNF (red) expression in NLC by confocal microscopy. ( c ) Relative expression of BDNF mRNA by normal healthy monocytes (n = 6) and NLC isolated from B-CLL patients (n = 6), as determined by RT-qPCR. ( d ) Representative western blots showing BDNF expression in supernatants of two independent NLC culture (n = 6). ( e ) Representative western blots showing BDNF expression by B-CLL cells cultured alone, with autologous NLC, or with autologous NLC plus an anti-BDNF blocking antibody (anti-hBDNF; 200 ng/mL) for 48 h. The three conditions for each patient assessed on the same western blot, which has been cropped to present only relevant data. The uncropped western blot membranes are shown in Supplementary Fig. ( f ) Quantification of BDNF protein from independent patient samples (n = 5). ( g ) Relative expression of BDNF mRNA by B-CLL cells cultured alone or with autologous NLC for 48 h, as determined by RT-qPCR (n = 7). ( h ) Flow cytometry analysis of cell death, as assessed by annexin V-fluorescein isothiocyanate/propidium iodide dual staining of B-CLL cells cultured for 72 h either alone, with autologous NLC, or with autologous NLC plus an anti-BDNF antibody (200 ng/mL). Cell death was assessed by excluding annexin V/propidium iodide-negative cells. Experiments were performed using n = 9 patient samples. Data are presented as the mean ± SEM from at least three independent experiments (* p < 0.05, ** p < 0.01, *** p < 0.001). ns not significant. Blots are cropped for clarity; full-length blots are shown in the Supplementary Fig. .

Article Snippet: BDNF was neutralized using an anti-BDNF mouse monoclonal antibody (200 ng/mL; #GF35L, Merck Millipore).

Techniques: Western Blot, Expressing, Immunofluorescence, Confocal Microscopy, Isolation, Quantitative RT-PCR, Cell Culture, Blocking Assay, Flow Cytometry, Staining

Journal: Scientific Reports

Article Title: BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells

doi: 10.1038/s41598-020-69307-1

Figure Lengend Snippet: The effects of BDNF on B-CLL pro-survival signaling are similar to those of BAFF, APRIL, and CXCL12 combined. ( a ) Representative western blot showing expression of NTSR2, p-Src, Src, and Bcl-2 by B-CLL cells isolated from patients. Cells were either co-cultured for 48 h with autologous NLC or cultured alone in complete medium supplemented (as indicated) with exogenous human (h) CXCL12 (100 ng/mL), BAFF (2 ng/mL), APRIL (25 ng/mL), or BDNF (100 ng/mL). (b,c,d), full-length blots are shown in the Supplementary Fig. . Quantification of NTSR2 ( b ), p-Src ( c ), and Bcl-2 ( d ) expression in four different patient samples. Data are presented as the mean ± SEM from at least three independent experiments, (* p < 0.05, ** p < 0.01). Blots are cropped for clarity; full blots are shown in the Supplementary Fig. .

Article Snippet: BDNF was neutralized using an anti-BDNF mouse monoclonal antibody (200 ng/mL; #GF35L, Merck Millipore).

Techniques: Western Blot, Expressing, Isolation, Cell Culture

Journal: Scientific Reports

Article Title: BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells

doi: 10.1038/s41598-020-69307-1

Figure Lengend Snippet: Inhibiting BDNF in addition to BAFF, APRIL, and CXCR4 reverses NLC-mediated protection of B-CLL cells from apoptosis. ( a ) Representative western blot showing expression of p-Src and Bcl-2 by B-CLL cells isolated from patients. Cells were cultured (for 72 h) alone, with autologous NLC, or with autologous NLC plus single or combined inhibition of BAFF (anti-hBAFF, 100 ng/mL), APRIL (anti-hAPRIL, 500 ng/mL), CXCL12 receptor CXCR4 (AMD3100, 0.5 µg/mL), and BDNF (anti-hBDNF, 200 ng/mL). ( b – d ), full-length blots are shown in the Supplementary Fig. .Quantification of NTSR2 ( b ), p-Src ( c ) and Bcl-2 ( d ) expression in six independent experiments using six different patient samples. ( e ) Flow cytometry analysis of cell death, assessed by annexin V-fluorescein isothiocyanate/propidium iodide dual staining of B-CLL cells cultured (for 72 h) either alone, with autologous NLC, or with autologous NLC and single or combined inhibition of BAFF (anti-hBAFF, 100 ng/mL), APRIL (anti-hAPRIL, 500 ng/mL), CXCL12 receptor CXCR4 inhibition (AMD3100, 0.5 µg/mL), and BDNF (anti-hBDNF, 200 ng/mL). Cell death was assessed by exclusion of annexin V/propidium iodide-negative cells. Experiments were performed using n = 9 different patient samples. Data are presented as the mean ± SEM from at least three independent experiments (* p < 0.05, ** p < 0.01, *** p < 0.001). ns not significant. ( f ) Schematic representation of the obtained results. NLC produce and secrete BDNF, which promotes survival of B-CLL cells by activating the Src signaling pathway and upregulating expression of Bcl-2. This newly described member of the NLC secretome appears to exert both complementary (alongside BAFF, APRIL and CXCL12) and independent effects. Here, we propose a model in which BDNF or pro-survival cytokines secreted by NLC within survival centers balance each other out to facilitate survival of B-CLL cells, and argue that simultaneous inhibition of BDNF signaling through the NTSR2-TrkB conditional oncogenic platform, along with inhibition of BAFF, APRIL and CXCR4/CXCL12, could cancel out NLC-mediated protection of B-CLL cells from apoptosis and restore normal cell death.

Article Snippet: BDNF was neutralized using an anti-BDNF mouse monoclonal antibody (200 ng/mL; #GF35L, Merck Millipore).

Techniques: Western Blot, Expressing, Isolation, Cell Culture, Inhibition, Flow Cytometry, Staining

Journal: Research in Pharmaceutical Sciences

Article Title: Aflatoxin G1 exposure altered the expression of BDNF and GFAP, histopathological of brain tissue, and oxidative stress factors in male rats

doi: 10.4103/1735-5362.359434

Figure Lengend Snippet: Nucleotide sequences of primers.

Article Snippet: Primary antibodies were rabbit anti-mouse for GFAP (Biocare, USA, Cas NO: SKU065) and BDNF (Creative Biolabs, USA, Cas NO: CBMAB-AP613LY).

Techniques:

Journal: Research in Pharmaceutical Sciences

Article Title: Aflatoxin G1 exposure altered the expression of BDNF and GFAP, histopathological of brain tissue, and oxidative stress factors in male rats

doi: 10.4103/1735-5362.359434

Figure Lengend Snippet: Quantitative evaluation of (A) GFAP and (B) BDNF gene expression based on GAPDH. Different groups received aflatoxin G1 at 2 mg/kg for 15, 28, and 45 days. Data are presented as mean ± SD. * P < 0.05 and *** P < 0.001 indicate significant differences compared to the control group. GFAP, Glial fibrillary acidic protein; BDNF, brain-derived neurotrophic factors.

Article Snippet: Primary antibodies were rabbit anti-mouse for GFAP (Biocare, USA, Cas NO: SKU065) and BDNF (Creative Biolabs, USA, Cas NO: CBMAB-AP613LY).

Techniques: Gene Expression, Control, Derivative Assay

Journal: Research in Pharmaceutical Sciences

Article Title: Aflatoxin G1 exposure altered the expression of BDNF and GFAP, histopathological of brain tissue, and oxidative stress factors in male rats

doi: 10.4103/1735-5362.359434

Figure Lengend Snippet: (A) Immunohistochemical staining of the cerebral cortex of the rats receiving aflatoxin G1 at 2 mg/kg for 15, 28, and 45 days in order to evaluation of GFAP protein expression. control group; (B) the graph is representing the relative expression of GFAP protein. In the control group, GFAP protein synthesis is seen in the cortical cell line (to a lesser extent), while in the experimental groups of 15, 28, and 45 days, the synthesis of this protein increased over time. In the group receiving aflatoxin G1 for 45 days, protein synthesis increased significantly. ** P < 0.01 and *** P < 0.001 indicate significant differences compared to the control group. GFAP, Glial fibrillary acidic protein.

Article Snippet: Primary antibodies were rabbit anti-mouse for GFAP (Biocare, USA, Cas NO: SKU065) and BDNF (Creative Biolabs, USA, Cas NO: CBMAB-AP613LY).

Techniques: Immunohistochemical staining, Staining, Expressing, Control